Difference between revisions of "Part:BBa K3722008"
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We used it to oxidate 6-Br-indole and produce 6,6’-dibromoindigo(Tyrian purple). | We used it to oxidate 6-Br-indole and produce 6,6’-dibromoindigo(Tyrian purple). | ||
In addition, MaFMO can oxidized indole to indigo. | In addition, MaFMO can oxidized indole to indigo. | ||
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===Usage=== | ===Usage=== |
Latest revision as of 00:27, 22 October 2021
T7_Promoter-RBS-MaFMO-T7_Terminator
An oxidation reaction enzyme that catalyzes the binding of oxygen atoms of molecular oxygen to the substrate,Monooxygenase hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.
Biology
MaFMO hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.
We used it to oxidate 6-Br-indole and produce 6,6’-dibromoindigo(Tyrian purple). In addition, MaFMO can oxidized indole to indigo.
Usage
We construct the composite part BBa_K3332052 and transformed it into E. coli BL21 (DE3) to verify its expression.
Characterization
The verification of the expression We used IPTG to induce the expression at different times.
- Fig.1 SDS-PAGE analysis of MaFMO .Induction condition: LB media, OD600 0.6 ~ 0.8, IPTG 0.1 mM, 18°C and 200 rpm.(0:negitive control 1:4h 2:5h 3:6h 4:7h 5:8h 6:9h 7:10h 8:11h)
References
Han, G. H., Shin, H. J. & Kim, S. W . Optimization of bio-indigo productionby recombinant E-coli harboring fmo gene. Enzyme. Micro. Tech. 42, 617–623 (2008).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1435
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 633
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 236
Illegal AgeI site found at 870 - 1000COMPATIBLE WITH RFC[1000]