Difference between revisions of "Part:BBa K3971006"
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[[File:T--IISER-Pune-India--bruschi.jpg|thumb|300x370px|centre|''csc'' operon and sucrose consumption pathway in ''E. coli'' EC3132. Figure adapted from Bruschi ''et al.'' (2011)]]
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[[File:T--IISER-Pune-India--bruschi.png|thumb|300x370px|centre|''csc'' operon and sucrose consumption pathway in ''E. coli'' EC3132. Figure adapted from Bruschi ''et al.'' (2011)]]
  
 
===References===
 
===References===

Revision as of 23:54, 21 October 2021


Bidirectional promoter from E. coli EC3132

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This is a native bidirectional promoter of the csc operon from E. coli EC3132, a strain that can naturally consume sucrose [1].

The csc operon consists of the genes cscA, cscB and cscK. cscA codes for sucrose-6-phosphate hydrolase, which hydrolyses sucrose in glucose and fructose. cscB codes for sucrose permease, a sucrose-proton symporter on bacterial cell membranes. cscK codes for fructokinase, which catalyses the conversion of fructose to fructose-6-phosphate [2].

cscB facilitates the import of sucrose into the bacterium, after which it is hydrolysed into glucose and fructose using the gene product of cscA, and then phosphorylated by the gene product of cscK[1].

The broader csc operon consists of two constituent operons, a cscA operon and a cscBK operon, both of which are under the bidirectional promoter. The cscABK genes are regulated by a repressor coded by cscR, which is under the control of a different promoter[2].

This bidirectional promoter shares a 99.55% sequence alignment with its homologue from E. coli W (verified by NCBI BLAST search), a strain that can also naturally consume sucrose.


csc operon and sucrose consumption pathway in E. coli EC3132. Figure adapted from Bruschi et al. (2011)

References

[1] Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., & Lengeler, J. W. (2002). Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132. Journal of bacteriology, 184(19), 5307–5316. https://doi.org/10.1128/JB.184.19.5307-5316.2002

[2] Bruschi, M., Boyes, S. J., Sugiarto, H., Nielsen, L. K., & Vickers, C. E. (2012). A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains. Biotechnology advances, 30(5), 1001-1010.