Difference between revisions of "Part:BBa K3971006"
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[2] Bruschi, M., Boyes, S. J., Sugiarto, H., Nielsen, L. K., & Vickers, C. E. (2012). A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains. Biotechnology advances, 30(5), 1001-1010. | [2] Bruschi, M., Boyes, S. J., Sugiarto, H., Nielsen, L. K., & Vickers, C. E. (2012). A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains. Biotechnology advances, 30(5), 1001-1010. | ||
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Revision as of 23:42, 21 October 2021
Bidirectional promoter from E. coli EC3132
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This is a native bidirectional promoter of the csc operon from E. coli EC3132, a strain that can naturally consume sucrose [1].
The csc operon consists of the genes cscA, cscB and cscK. cscA codes for sucrose-6-phosphate hydrolase, which hydrolyses sucrose in glucose and fructose. cscB codes for sucrose permease, a sucrose-proton symporter on bacterial cell membranes. cscK codes for fructokinase, which catalyses the conversion of fructose to fructose-6-phosphate [2].
cscB facilitates the import of sucrose into the bacterium, after which it is hydrolysed into glucose and fructose using the gene product of cscA, and then phosphorylated by the gene product of cscK[1].
The broader csc operon consists of two constituent operons, a cscA operon and a cscBK operon, both of which are under the bidirectional promoter. The cscABK genes are regulated by a repressor coded by cscR, which is under the control of a different promoter[2].
This bidirectional promoter shares a 99.55% sequence alignment with its homologue from E. coli W (verified by NCBI BLAST search), a strain that can also naturally consume sucrose.
References
[1] Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., & Lengeler, J. W. (2002). Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132. Journal of bacteriology, 184(19), 5307–5316. https://doi.org/10.1128/JB.184.19.5307-5316.2002
[2] Bruschi, M., Boyes, S. J., Sugiarto, H., Nielsen, L. K., & Vickers, C. E. (2012). A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains. Biotechnology advances, 30(5), 1001-1010.