Difference between revisions of "Part:BBa K3792003"
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<partinfo>BBa_K3792003 short</partinfo>
 
<partinfo>BBa_K3792003 short</partinfo>
  
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apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.  
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<p>
 +
apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.<br>
  
−
This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.  
+
This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEBExpress.<br>
  
−
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
+
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.<br>
 +
</p>
  
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<table id="modular-promoter-library-chart" class="charts-css column hide-data show-primary-axis show-10-secondary-axes">
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<div id="my-chart">
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<table id="modular-promoter-library-chart" class="charts-css column show-labels show-data show-primary-axis show-10-secondary-axes">
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      <caption>Promoter Strength Measurement</caption>
 
       <tr>
 
       <tr>
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         <td style="--size: calc(897/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_M36303 BBa_M36303]<br>BIOFAB: apFAB46<br>Strength: 897</span><span class="data">897</span></td>
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        <th scope="row">apFAB40<br>BBa_K3792003</th>
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         <td style="--size: calc(2.477/2.5)"><span class="tooltip">Supernatant of the Chitin Secretion Cells</span><span class="data">2.477</span></td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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       <td style="--size: calc(866.7/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_K2832101 BBa_K2832101]<br>BIOFAB: apFAB70<br>Strength: 866.7</span><span class="data">866.7</span></td>
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      <th scope="row">LB and Assay</th>
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       <td style="--size: calc(1.515/2.5)"><span class="tooltip">LB Growth Medium with the Chitosan Detection Assay</span><span class="data">1.515</span></td>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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         <td style="--size: calc(866/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_K2832102 BBa_K2832102]<br>BIOFAB: apFAB71<br>Strength: 866</span><span class="data">866</span></td>  
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        <th scope="row">DI Water and Assay</th>
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         <td style="--size: calc(0.084/2.5)"><span class="tooltip">Deionized Water with the Chitosan Assay</span><span class="data">0.084</span></td>  
 
       </tr>
 
       </tr>
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       <tr>       
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       <tr>
−
         <td style="--size: calc(857/900)"><span class="tooltip">iGEM: [https://parts.igem.org/Part:BBa_K2832103 BBa_K2832103]<br>BIOFAB: apFAB61<br>Strength: 857</span><span class="data">857</span></td>
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        <th scope="row">LB and No Assay</th>       
 +
         <td style="--size: calc(0.08/2.5)"><span class="tooltip">LB Growth Medium with No Chitosan Assay</span><span class="data">0.08</span></td>
 
     </tr>
 
     </tr>
 
   </table>
 
   </table>
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  <div class="data-axis">Absorbance of 540 nm Light</div>
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</div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:06, 22 October 2021


BIOFAB apFAB40 Measurement Device

apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.
This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEBExpress.
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.

Promoter Strength Measurement
apFAB40
BBa_K3792003		 Supernatant of the Chitin Secretion Cells2.477
LB and Assay LB Growth Medium with the Chitosan Detection Assay1.515
DI Water and Assay Deionized Water with the Chitosan Assay0.084
LB and No Assay LB Growth Medium with No Chitosan Assay0.08
Absorbance of 540 nm Light

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]