Difference between revisions of "Part:BBa K3890004"
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Figure 2. Schematic representation of the novel CYP6G1 fused to NADPH-dependent cytochrome P450 reductase (CPR) by the flexible linker (GGGGS)x5. The N-terminal sequence of the membrane-anchoring CPR was removed to support the protein movement and rising the electron transfer efficiency. CYP6G1, cytochrome P450 monooxygenase; CPR, cytochrome P450 reductase; FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide; NADPH, reduced nicotinamide adenine dinucleotide phosphate. | Figure 2. Schematic representation of the novel CYP6G1 fused to NADPH-dependent cytochrome P450 reductase (CPR) by the flexible linker (GGGGS)x5. The N-terminal sequence of the membrane-anchoring CPR was removed to support the protein movement and rising the electron transfer efficiency. CYP6G1, cytochrome P450 monooxygenase; CPR, cytochrome P450 reductase; FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide; NADPH, reduced nicotinamide adenine dinucleotide phosphate. | ||
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The artificial fusion of both parts through this linker reduces the molar ratio from 15:1 to 1:1 between CPR and CYP6G1, respectively [4]. This means a higher electron transfer efficiency from the NADPH cofactor to the CYP6G1 enzyme, promoting much higher enzymatic turnover rates compared to the unfused CYP6G1 [4]. Consequently, this high turnover rate allows the enzyme to efficiently catalyze (e.g., hydroxylate) a greater amount of the substrate (i.e, imidacloprid) thus improving the enzymatic catalysis of CYP6G1. | The artificial fusion of both parts through this linker reduces the molar ratio from 15:1 to 1:1 between CPR and CYP6G1, respectively [4]. This means a higher electron transfer efficiency from the NADPH cofactor to the CYP6G1 enzyme, promoting much higher enzymatic turnover rates compared to the unfused CYP6G1 [4]. Consequently, this high turnover rate allows the enzyme to efficiently catalyze (e.g., hydroxylate) a greater amount of the substrate (i.e, imidacloprid) thus improving the enzymatic catalysis of CYP6G1. | ||
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Revision as of 23:42, 21 October 2021
CYP6G1 fusioned with NADPH reductase
CYP6G1 fusioned with NADPH reductase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1947
Illegal BglII site found at 3291 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 711
Illegal BsaI.rc site found at 341
Illegal BsaI.rc site found at 3242
Illegal SapI.rc site found at 1822