Difference between revisions of "Part:BBa K3725050"
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<partinfo>BBa_K3725050 short</partinfo>
 
<partinfo>BBa_K3725050 short</partinfo>
  
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The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. In the presence of the <i>P. Cryptogea</i> X24 gene, the hairpin loop will unfold and express the downstream GFP reporter protein.  
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The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of Phytophthora cryptogea, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease P. cryptogea.
  
  

Revision as of 03:47, 18 December 2021


Phytophthora Toehold Switch Sequence

The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of Phytophthora cryptogea, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease P. cryptogea.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]