Difference between revisions of "Part:BBa K3805238"
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<h2>'''Usage and Biology'''</h2>
 
<h2>'''Usage and Biology'''</h2>
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<p>Anti-mCherry pmrB is the modified extracelluar receptor in the PmrBAC system in workers. PmrBAC is the external signal sensing system with extracelluar receptor pmrB, intrcelluar signal pmrA and the promoter of the aimed gene pmrC. In our system, the Iron-binding motif of pmrB is replaced by part of Anti-mCherry sequence. When the worker becomes the cheater, the extermination system is open and the secreted AIP binds to guard which activates the expression of mCherry. Afterwards, with the interaction between anti-mCherry pmrB and mCherry outside of the cheaters, pmrA phosphorylates and activates the promoter pmrC, regulating the transcription of its downstream endotoxin gene ccdb. </p>
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<p>Anti-mCherry pmrB is the modified extracelluar receptor in the PmrBAC system in workers. PmrBAC is the external signal sensing system with extracelluar receptor pmrB, intrcelluar signal pmrA and the promoter of the aimed gene pmrC. In our system, the Iron-binding motif of pmrB is replaced by part of anti-mCherry sequence. When the worker becomes the cheater, the extermination system is open and the secreted AIP binds to guard which activates the expression of mCherry. Afterwards, with the interaction between anti-mCherry pmrB and mCherry outside of the cheaters, pmrA phosphorylates and activates the promoter pmrC, regulating the transcription of its downstream endotoxin gene ccdb. </p>
 
<p>This part can be used to make the bacteria respond to any proteins linked to mCherry and enable the transcription of the target gene.
 
<p>This part can be used to make the bacteria respond to any proteins linked to mCherry and enable the transcription of the target gene.
 
[[File: Anti-mCherry PmrB.png|center|thumb|400px|
 
[[File: Anti-mCherry PmrB.png|center|thumb|400px|

Revision as of 23:16, 21 October 2021


anti-mCherry pmrB

This parts replaces the extracellular binding domain of the receptor pmrB in the PmrBAC system with an antibody sequence that binds to mCherry, enabling it to open a downstream pathway by binding to mCherry. So we can use this part to make the bacteria respond to the proteins that are linked to mCherry.


Usage and Biology

Anti-mCherry pmrB is the modified extracelluar receptor in the PmrBAC system in workers. PmrBAC is the external signal sensing system with extracelluar receptor pmrB, intrcelluar signal pmrA and the promoter of the aimed gene pmrC. In our system, the Iron-binding motif of pmrB is replaced by part of anti-mCherry sequence. When the worker becomes the cheater, the extermination system is open and the secreted AIP binds to guard which activates the expression of mCherry. Afterwards, with the interaction between anti-mCherry pmrB and mCherry outside of the cheaters, pmrA phosphorylates and activates the promoter pmrC, regulating the transcription of its downstream endotoxin gene ccdb.

This part can be used to make the bacteria respond to any proteins linked to mCherry and enable the transcription of the target gene.

Figure 1: Anti-mCherry pmrB.

Improvement

This part helps to extend the using areas of pmrBAC system, for it can respond to any proteins which are linked with mCherry and activates the expression of any target genes we want. Therefore, it improves the use efficiency and convenience of external sensing system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]