Difference between revisions of "Part:BBa K4059022:Design"

(→‎Design Notes)
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===Source===
 
===Source===
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MALLLAVF source:
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https://pubmed.ncbi.nlm.nih.gov/23886957/
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835717/
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Aromatic rings in the transmembrane regions:
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https://www.nature.com/articles/s41598-019-48541-2
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===References===
 
===References===

Latest revision as of 22:04, 21 October 2021


CPR from Phanerochaete chrysosporium with N-terminal substitution (2), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 227
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the chimera N-terminals which gave the best expression level from the referenced study

Source

https://pubmed.ncbi.nlm.nih.gov/23886957/

References