Difference between revisions of "Part:BBa K4059022:Design"
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===Source=== | ===Source=== | ||
| + | MALLLAVF source: | ||
| + | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835717/ | ||
| + | |||
| − | . | + | Aromatic rings in the transmembrane regions: |
| + | https://www.nature.com/articles/s41598-019-48541-2 | ||
===References=== | ===References=== | ||
Revision as of 21:59, 21 October 2021
CPR from Phanerochaete chrysosporium with N-terminal substitution (2), E. coli K12 codon optimized
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 227
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Inspirered of the must commom point mutation ”MALLLAVF” a similar mutation has been introduced in the original N-terminal for PsiH.
Source
MALLLAVF source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835717/
Aromatic rings in the transmembrane regions:
https://www.nature.com/articles/s41598-019-48541-2