Difference between revisions of "Part:BBa K3725110"

Revision as of 21:50, 21 October 2021

PhoA Promoter with GFP Reporter

DESIGN

The inducible promoter BBa_K1682012, created by 2015 HKUST-Rice iGEM, was originally made to control the expression of the PhoA alkaline phosphatase gene following activation by the phosphorylated PhoB transcription factor. By replacing the downstream PhoA gene with GFP, the part expresses fluorescence in the presence of low levels of extracellular phosphate.

EXPERIENCE

Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K3725110 phosphate sensor. However, the characterization data (Figure 1) yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.

Figure 5: Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM.