Difference between revisions of "Part:BBa K3783003:Experience"
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<figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption> | <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption> | ||
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| + | <p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin. | ||
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OhioState Application
The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.
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