Difference between revisions of "Part:BBa K3861005"
Line 9: Line 9:
 
</html>
 
</html>
  
−
==='''Biology'''===
+
==='''Usage and Biology'''===
  
 
<html>
 
<html>
 +
 +
This backbone was used for the characterisation of the PlldR promoter (<a href="https://parts.igem.org/Part:BBa_BBa_K1847008">BBa_BBa_K1847008</a>) with the GFP reporter (<a href="https://parts.igem.org/Part:BBa_BBa_E0840">BBa_BBa_E0840</a>) in <I>Salmonella</I> Typhimurium.
 +
This backbone is intended to be used in the composite part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">BBa_K3861026</a>.
 +
 +
 +
 
<figure>
 
<figure>
 
<img src="https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png" alt="pKF01" width="350">
 
<img src="https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png" alt="pKF01" width="350">
Line 21: Line 27:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
We used this backbone as part of the composite part / backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">BBa_K3861026</a>.
+
 
 +
 
 +
 
 +
 
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K3861005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3861005 SequenceAndFeatures</partinfo>
  

Revision as of 21:24, 21 October 2021


pKF01

Introduction

iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of thyA (BBa_K3861003) and lldR (BBa_K3861004). The expression of thyA and lldR is driven by the synthetic constitutive promoter P_syn-thyA (BBa_K3861002) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF.

Usage and Biology

This backbone was used for the characterisation of the PlldR promoter (BBa_BBa_K1847008) with the GFP reporter (BBa_BBa_E0840) in Salmonella Typhimurium. This backbone is intended to be used in the composite part BBa_K3861026.

pKF01
Fig. 1: Coloring of Salmonella Typhimurium colonies harboring either PlldR-GFP inserted in pKF01 vector (plate on top) or in pSB1C3 vector (plate below).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
    Illegal XhoI site found at 2804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]