Difference between revisions of "Part:BBa K3722004"
| Line 8: | Line 8: | ||
===Biology=== | ===Biology=== | ||
It is a rigid peptide, which is used to completely isolate the two connected proteins and maintain their independent functions, because some flexible peptides will cause the expression of the fusion protein to decrease or the activity to be lost. It should be noted that rigid peptides cannot be used for two interacting proteins. | It is a rigid peptide, which is used to completely isolate the two connected proteins and maintain their independent functions, because some flexible peptides will cause the expression of the fusion protein to decrease or the activity to be lost. It should be noted that rigid peptides cannot be used for two interacting proteins. | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://2021.igem.org/wiki/images/e/ee/T--NWU-CHINA-A--part04.png" width="100%" style="float:center"> | ||
| + | <figcaption> | ||
| + | <p style="font-size:1rem"> | ||
| + | </p> | ||
| + | </figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
Revision as of 21:16, 21 October 2021
A linker sequence in fusion protein Fre-Linker-SttH
A linker sequence belongs to our fusion protein Fre-Linker-SttH, coding a rigid linker between Fre and SttH.
Biology
It is a rigid peptide, which is used to completely isolate the two connected proteins and maintain their independent functions, because some flexible peptides will cause the expression of the fusion protein to decrease or the activity to be lost. It should be noted that rigid peptides cannot be used for two interacting proteins.
Usage
The part was used to construct SttH and Fre fusion enzyme to enhance the solubility and activity of SttH. Due to the limited time, we did not characterize this part. As a result, there is a lack of data about this part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]