Difference between revisions of "Part:BBa K4041001"
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| − | <partinfo>BBa_K4041001 short</partinfo><br> | + | <partinfo>BBa_K4041001 short</partinfo><br><br> |
| − | This make the composite part https://parts.igem.org/Part:BBa_K4041004 <br> | + | This make the composite part https://parts.igem.org/Part:BBa_K4041004 <br><br> |
This is a non-coding part that consist of the ctxA gene of the Vibrio Cholerae. The genome template is used for cloning into a plasmid and contains conserved regions so teams could design detection kit based on the conserved regions. | This is a non-coding part that consist of the ctxA gene of the Vibrio Cholerae. The genome template is used for cloning into a plasmid and contains conserved regions so teams could design detection kit based on the conserved regions. | ||
Latest revision as of 20:32, 21 October 2021
Vibrio Cholerae partial genomic ctxA template
This make the composite part https://parts.igem.org/Part:BBa_K4041004
This is a non-coding part that consist of the ctxA gene of the Vibrio Cholerae. The genome template is used for cloning into a plasmid and contains conserved regions so teams could design detection kit based on the conserved regions.
ctxA gene is one of the central and important gene that makes the Vibrio Cholerae pathogenic. While it is important for its cytotoxicity, it itself could be served as a biomarker for the strains.
| The following qPCR primer were proved to work in this block in in Vibrio | |
| TDH Reverse | GACACCGCTGCCATTGTATAGT |
| TDH Forward | CCCGGTTCTGATGAGATATTGT |
| vcgC Reverse | CGCCAATAGCCTGTTCAGAT |
| vcgC Forward | GCAGTTCAAACATGGTCTCAAA |
| ctxA Reverse | GGTCTTATGCCAAGAGGACAGA |
| ctxA Forward | CCATCATCGTGCCTAACAAATC |
| The following RPA primer were proved to work in this block in in Vibrio | |
| TDH Reverse | CTTATAGCCAGACACCGCTGCCATTGTATAGT |
| TDH Forward | CCCGGTTCTGATGAGATATTGTTTGTTGTTCG |
| vcgC Reverse | GCGAGTAGTGAGCCGATCCCGCCAATAGCCTG |
| vcgC Forward | GGCGCAGTTCAAACATGGTCTCAAAAAGGAGC |
| ctxA Reverse | GCAGTCAGGTGGTCTTATGCCAAGAGGACAGA |
| ctxA Forward | ACATATCCATCATCGTGCCTAACAAATCCCGT |
| The following crRNA were proved to work in this block in in Vibrio | |
| TDH forward (crRNA1) | ACTGTGAACATTAATGATAA |
| vcgC forward (crRNA3) | ATAGCACTAATGTGTCATCT |
| vcgC reverse (crRNA4, with PAM) | GCGGAGACGAGATCGCTATC |
| ctxA Forward (crRNA5) | TGATCATGCAAGAGGAACTC |
| crRNA 2 (high background) and crRNA 6 (no cleavage signal) is not functional from design, and is discarded |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]