Difference between revisions of "Part:BBa K3861005"
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iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of <I>thyA</I> (<a href="https://parts.igem.org/Part:BBa_K3861003">BBa_K3861003</a>) and <I>lldR</I>  (<a href="https://parts.igem.org/Part:BBa_K3861004">BBa_K3861004</a>). The expression of <I>thyA</I> and <I>lldR</I> is driven by the synthetic constitutive promoter P_syn-thyA (<a href="https://parts.igem.org/Part:BBa_K3861002">BBa_K3861002</a>) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF.
 
iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of <I>thyA</I> (<a href="https://parts.igem.org/Part:BBa_K3861003">BBa_K3861003</a>) and <I>lldR</I>  (<a href="https://parts.igem.org/Part:BBa_K3861004">BBa_K3861004</a>). The expression of <I>thyA</I> and <I>lldR</I> is driven by the synthetic constitutive promoter P_syn-thyA (<a href="https://parts.igem.org/Part:BBa_K3861002">BBa_K3861002</a>) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF.
  
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We used this backbone as part of the composite part / backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">BBa_K3861026</a>.
 
 
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<figure>
 
<figure>
 
<img src="https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png" alt="pKF01" width="350">
 
<img src="https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png" alt="pKF01" width="350">
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<figcaption>Fig. 1 - GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).</figcaption>
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<figcaption>Fig. 1: Coloring of Salmonella Typhimurium colonies harboring either PlldR-GFP inserted in pKF01 vector (plate on top) or in pSB1C3 vector (plate below).</figcaption>
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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We used this backbone as part of the composite part / backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">BBa_K3861026</a>.
  
 
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Revision as of 21:15, 21 October 2021


pKF01

Introduction

iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of thyA (BBa_K3861003) and lldR (BBa_K3861004). The expression of thyA and lldR is driven by the synthetic constitutive promoter P_syn-thyA (BBa_K3861002) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF.

Biology

pKF01
Fig. 1: Coloring of Salmonella Typhimurium colonies harboring either PlldR-GFP inserted in pKF01 vector (plate on top) or in pSB1C3 vector (plate below).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
    Illegal XhoI site found at 2804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]