Difference between revisions of "Part:BBa K3833007"
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Our team used PCR to amplify our composite Scl2 with fibronectin binding domain gBlock with our custom primers. After amplification, a gel electrophoresis was run on the PCR product using 1Kb Plus DNA Ladder. A significant band was obtained for the PCR, around 1,400bp (Picture 1). Because we predicted this composite part to be 1,394 bp, it was determined that amplification of our Scl2 with fibronectin binding domain successfully occurred.
 
Our team used PCR to amplify our composite Scl2 with fibronectin binding domain gBlock with our custom primers. After amplification, a gel electrophoresis was run on the PCR product using 1Kb Plus DNA Ladder. A significant band was obtained for the PCR, around 1,400bp (Picture 1). Because we predicted this composite part to be 1,394 bp, it was determined that amplification of our Scl2 with fibronectin binding domain successfully occurred.
  
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[File:Fibronectin Gel.png|500px|center|thumb|Img 1. Gel Electrophoresis (1Kb Plus Ladder) of Part:BBa_K3833007 — Scl2 with Fibronectin Binding Domain PCR Product]]
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[[File:Fibronectin Gel.png|500px|center|thumb|Img 1. Gel Electrophoresis (1Kb Plus Ladder) of Part:BBa_K3833007 — Scl2 with Fibronectin Binding Domain PCR Product]]
  
  

Revision as of 20:11, 21 October 2021


Scl2 with Fibronectin Binding Domain

Fibronectin is a glycoprotein found in the extracellular matrix. It binds to receptor proteins such as integrins, collagen, fibrin, and other proteins. Fibronectin plays a large role in cell adhesion, growth, and differentiation, and is crucial to wound healing. By integrating fibronectin into Scl2, scaffolds made of Scl2 can accommodate cells with fibronectin binding sites.

Our team used PCR to amplify our composite Scl2 with fibronectin binding domain gBlock with our custom primers. After amplification, a gel electrophoresis was run on the PCR product using 1Kb Plus DNA Ladder. A significant band was obtained for the PCR, around 1,400bp (Picture 1). Because we predicted this composite part to be 1,394 bp, it was determined that amplification of our Scl2 with fibronectin binding domain successfully occurred.

Img 1. Gel Electrophoresis (1Kb Plus Ladder) of Part:BBa_K3833007 — Scl2 with Fibronectin Binding Domain PCR Product


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1307
  • 1000
    COMPATIBLE WITH RFC[1000]