Difference between revisions of "Part:BBa K3739016"
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===Usage===
 
===Usage===
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By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''BL21(DE3)'' was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter, and the constructed plasmid was transformed into ''BL21(DE3)'' to verify its expression.<br/>
+
By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter, and the constructed plasmid was transformed into BL21(DE3) to verify its expression.<br/>
  
  

Revision as of 23:07, 21 October 2021


AKTA-his-Mfp5

It is a kind of mucin from mussels, including AKTA expression tags and 6xHis affinity tags which can let the mucin purified by tags.

Biology

Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.

Usage

By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter, and the constructed plasmid was transformed into BL21(DE3) to verify its expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]