Difference between revisions of "Part:BBa K3714010"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3714010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3714010 SequenceAndFeatures</partinfo> | ||
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| + | <center>[[File:K3714016.png]]</center> | ||
| + | <center><b>Figure.1 | Verification of temporarily inhibition and disinhibition by oligonucleotides. </b>The aptazyme was produced by a T7 <i>in vitro</i> transcription system. The product was run on a denatured PAGE. Gardiquimod is an inhibitor of the cleavage activity. "stop" and "start" are two oligonucleotides designed to control the activity of the aptazyme/</center> | ||
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Revision as of 02:15, 22 October 2021
Improved gard-337 for lateral flow assay
To test our optimization design with TMSD, a structureless toehold site between biotin probe binding site and aptazyme domain was need. However, designing the probe with manual work and test with available RNA structure prediction model is such a huge work with plenty of attempts. This part is designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed probe shows no influence on the function of aptazyme, the toehold worked as our expect as well.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
