Difference between revisions of "Part:BBa K3739027"
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<partinfo>BBa_K3739027 short</partinfo> | <partinfo>BBa_K3739027 short</partinfo> | ||
| − | We anchor | + | We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use <partinfo>BBa_K3739057</partinfo> to construct the expression system and anchor LCIKR-2 on the surface of VnDX. |
| − | === | + | ===Biology=== |
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX. | LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from ''Bacillus subtilis'', can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX. | ||
| + | ===Usage=== | ||
| + | |||
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Revision as of 19:56, 21 October 2021
Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
to construct the expression system and anchor LCIKR-2 on the surface of VnDX.
Biology
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.
Usage
Sequence and Features Status: 500 Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.