Difference between revisions of "Part:BBa K4053001:Design"

(→‎Design Notes)
(→‎Design Notes)
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Data<br>
 
Data<br>
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https://2021.igem.org/wiki/images/9/97/T--NTU-Singapore--brafv600efq.png
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https://2021.igem.org/wiki/images/b/b0/T--NTU-Singapore--FQdata.jpg
  
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<br>Graph of normalized signal ratio Mutant to Wild-Type with 1X Lamp product (blue) and 50X diluted lamp product (orange) for BRAF-V600 (left) and BRAF-E600 (right) after undergoing LAMP and FQ reaction.
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<br>Signal ratio of mutant to wild type BRAF gene tested with WT-LbCas12a and LbCas12aRVRR.
  
 
===Source===
 
===Source===

Revision as of 19:28, 21 October 2021


LBCas12a-RVRR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
    Illegal NotI site found at 3745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3123
    Illegal XhoI site found at 3754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
    Illegal NgoMIV site found at 3699
    Illegal NgoMIV site found at 3718
    Illegal AgeI site found at 3427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- Data
T--NTU-Singapore--FQdata.jpg


Signal ratio of mutant to wild type BRAF gene tested with WT-LbCas12a and LbCas12aRVRR.

Source

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References