Difference between revisions of "Part:BBa K4080012:Design"
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All of the parts are from the previous iGEM team that registered in the parts registry. | All of the parts are from the previous iGEM team that registered in the parts registry. | ||
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Latest revision as of 18:59, 21 October 2021
Sec system (PhoA), TEV protease with T7 expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- The system works under the T7 expression system, each part must be compatible and build strong-good relations
- The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
- Addition of 6xHis-tag after TEV protease
- Linkers placed between parts : GGG or GSG
Source
All of the parts are from the previous iGEM team that registered in the parts registry.