Difference between revisions of "Part:BBa K3794000"
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| − | Perna viridis foot protein-5 (PVFP-5), is one of the first adhesive proteins secreted by the mussel foot from the <i> Perna viridis </i> mussel species. | + | <p> Perna viridis foot protein-5 (PVFP-5), is one of the first adhesive proteins secreted by the mussel foot from the <i> Perna viridis </i> mussel species. </p> |
| − | PVFP-5 is an adhesive protein that can be utilised in biomedical and therapeutic applications due to its low immunogenicity and non-cytotoxicity in the body. It can be used as a bioadhesive coating for medical implants, such as a 3D printed scaffold / nerve channel for axonal regeneration. | + | <p> PVFP-5 is an adhesive protein that can be utilised in biomedical and therapeutic applications due to its low immunogenicity and non-cytotoxicity in the body. It can be used as a bioadhesive coating for medical implants, such as a 3D printed scaffold / nerve channel for axonal regeneration. </p> |
| − | There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation. | + | <p> There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation. </p> |
| − | This part has been synthesised by IDT as a composite, which can be found at <a href="parts.igem.org/Part:BBa_K3794001"> BBa_K3794001 </a> (with an upstream LacI Regulator (BBa_R0010)). | + | <p>This part has been synthesised by IDT as a composite, which can be found at <a href="parts.igem.org/Part:BBa_K3794001"> BBa_K3794001 </a> (with an upstream LacI Regulator (BBa_R0010)). </p> |
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Latest revision as of 18:10, 21 October 2021
PVFP-5
Perna viridis foot protein-5 (PVFP-5), is one of the first adhesive proteins secreted by the mussel foot from the Perna viridis mussel species.
PVFP-5 is an adhesive protein that can be utilised in biomedical and therapeutic applications due to its low immunogenicity and non-cytotoxicity in the body. It can be used as a bioadhesive coating for medical implants, such as a 3D printed scaffold / nerve channel for axonal regeneration.
There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.
This part has been synthesised by IDT as a composite, which can be found at BBa_K3794001 (with an upstream LacI Regulator (BBa_R0010)).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]