Difference between revisions of "Part:BBa K3861005"
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==='''Biology'''=== | ==='''Biology'''=== | ||
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| + | <img src=”https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png” alt=”pKF01” width=”511”> | ||
| + | <figcaption>Fig. 1 - GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).</figcaption> | ||
| + | </figure> | ||
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Revision as of 18:06, 21 October 2021
pKF01
Introduction
iGEM Humboldt_Berlin 2021 standard backbone based on pSB1C3. It contains the standard pSB1C3 ori, BBa_K3861003 and BBa_K3861004. We used this backbone as part of the composite part / backbone BBa_K3861026.
Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 997
Illegal XhoI site found at 2804 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]