Difference between revisions of "Part:BBa K3861005"
Line 12: Line 12:
 
<html>
 
<html>
 
<figure>
 
<figure>
−
<file src=”https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png” alt=”pKF01” width=”500”>
+
<source src=”https://2021.igem.org/wiki/images/a/a9/T--Humboldt_Berlin--pKF01.png” type="image/png" alt=”pKF01” width=”500”>
 
<figcaption>GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).</figcaption>
 
<figcaption>GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).</figcaption>
 
</figure>
 
</figure>

Revision as of 17:54, 21 October 2021


pKF01

Introduction

iGEM Humboldt_Berlin 2021 standard backbone based on pSB1C3. It contains the standard pSB1C3 ori, BBa_K3861003 and BBa_K3861004. We used this backbone as part of the composite part / backbone BBa_K3861026.

Biology

GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
    Illegal XhoI site found at 2804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]