Difference between revisions of "Part:BBa K4059009:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
This is a inducible promoter system, which is a mutant T7 Lac promoter.  The T7 promoter system allows induction of gene expression with isopropyl-β-D-1-thiogalactopyranoside (IPTG) in E. coli strains with the bacteriophage T7-polymerase in their genome (such as ER2566 or in general BL21(DE3) derivates). The mutant T7 Lac promoter is to be used together with the lacI, encoding the LacI repressor protein.
 
This is a inducible promoter system, which is a mutant T7 Lac promoter.  The T7 promoter system allows induction of gene expression with isopropyl-β-D-1-thiogalactopyranoside (IPTG) in E. coli strains with the bacteriophage T7-polymerase in their genome (such as ER2566 or in general BL21(DE3) derivates). The mutant T7 Lac promoter is to be used together with the lacI, encoding the LacI repressor protein.
 +
Consideration: There is choosen a medium inducible promoter system based on the former litterature where a high inducible promoter will not work in the cells.
  
 
===Source===
 
===Source===

Revision as of 16:42, 21 October 2021


T7Lac Promotor and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a inducible promoter system, which is a mutant T7 Lac promoter. The T7 promoter system allows induction of gene expression with isopropyl-β-D-1-thiogalactopyranoside (IPTG) in E. coli strains with the bacteriophage T7-polymerase in their genome (such as ER2566 or in general BL21(DE3) derivates). The mutant T7 Lac promoter is to be used together with the lacI, encoding the LacI repressor protein. Consideration: There is choosen a medium inducible promoter system based on the former litterature where a high inducible promoter will not work in the cells.

Source

Source: https://pubmed.ncbi.nlm.nih.gov/31550507/ and https://www.nature.com/articles/srep11301

References