Difference between revisions of "Part:BBa K3783008:Experience"
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===Applications of BBa_K3783008=== | ===Applications of BBa_K3783008=== | ||
| + | =OhioState Application= | ||
| + | <html> | ||
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| + | .generalimage { | ||
| + | display: block; | ||
| + | width: 80%; | ||
| + | margin: auto; | ||
| + | } | ||
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| + | </style> | ||
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| + | <body> | ||
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| + | <figure> | ||
| + | <img src="https://2021.igem.org/wiki/images/b/b0/T--OhioState--LALAssayGraph.png" class="generalimage" alt="LAL Assay Graph"> | ||
| + | <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption> | ||
| + | </figure> | ||
| + | <p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:30, 21 October 2021
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Applications of BBa_K3783008
OhioState Application
The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control
User Reviews
UNIQf89c03ad9c78ebea-partinfo-00000001-QINU UNIQf89c03ad9c78ebea-partinfo-00000002-QINU