Difference between revisions of "Part:BBa K3764002"
| Line 4: | Line 4: | ||
This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG). | This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG). | ||
| + | |||
| + | |||
It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764002 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications. | It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764002 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | |||
<!-- --> | <!-- --> | ||
Revision as of 16:25, 21 October 2021
mRFP1 with T38stop (TAG) mutation
This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).
It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764002 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]