Difference between revisions of "Part:BBa K3814004"
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| − | fuGFP, short for ‘free-use GFP’, is | + | fuGFP, short for ‘free-use GFP’, is an open source GFP developed by Mark Somerville and Nick Coleman at the University of Sydney. Current GFPs like superfolding GFP (sfGFP) are protected IP and patented. This can make any commercial or even academic use of them expensive, and a barrier to research. Reading through the patents of GFPs, it was realised that if a GFP could be made with less than 80% homology to currently patented GFPs, it would escape patent protection! |
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| − | Current GFPs like superfolding GFP (sfGFP) are protected IP and patented. This can make any commercial or even academic use of them expensive, and a barrier to research. Reading through the patents of GFPs, it was realised that if a GFP could be made with less than 80% homology to currently patented GFPs, it would escape patent protection! | + | |
So off they worked, and eventually they came to a product that had “76% amino acid identity to GFPmut3”, well below the 80% goal! See below its fluorescence: | So off they worked, and eventually they came to a product that had “76% amino acid identity to GFPmut3”, well below the 80% goal! See below its fluorescence: | ||
Revision as of 04:30, 14 June 2022
free-use GFP (fuGFP)
fuGFP, short for ‘free-use GFP’, is an open source GFP developed by Mark Somerville and Nick Coleman at the University of Sydney. Current GFPs like superfolding GFP (sfGFP) are protected IP and patented. This can make any commercial or even academic use of them expensive, and a barrier to research. Reading through the patents of GFPs, it was realised that if a GFP could be made with less than 80% homology to currently patented GFPs, it would escape patent protection!
So off they worked, and eventually they came to a product that had “76% amino acid identity to GFPmut3”, well below the 80% goal! See below its fluorescence:
Figure 1. Expression of fuGFP in E.coli strain TOP10.
References
Coleman, N., & Somerville, M. (2019, May). The Story of Free Use GFP (fuGFP). Small Things Considered. https://schaechter.asmblog.org/schaechter/2019/05/the-story-of-free-use-gfp-fugfp.html
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
- 1000COMPATIBLE WITH RFC[1000]