Difference between revisions of "Part:BBa K3814002"
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{| | {| | ||
| − | |Promoter | + | |'''Promoter''' |
| − | | | + | |'''-35''' |
| − | | | + | |'''-10''' |
|- | |- | ||
|Psal | |Psal | ||
Revision as of 14:01, 21 October 2021
Psal1 (PsalTTC, 100% Activity)
Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced.
| Promoter | -35 | -10 |
| Psal | TTATCA | TATCGT |
| PsalTTC | TTATTT | TACCGT |
This example showcases a variant that yields 34% expression levels of WT Psal promoters. This is described more indepth in the design section.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 68
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]