Difference between revisions of "Part:BBa K3747301"
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==References==
 
==References==
 
1. Y. Chen, J. M. L. Ho, D. L. Shis, et al., Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors. Nat. Commun. 9, 1–8 (2018).
 
1. Y. Chen, J. M. L. Ho, D. L. Shis, et al., Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors. Nat. Commun. 9, 1–8 (2018).

Revision as of 13:18, 21 October 2021


Plux/lac_dE

Plux/lac_dE hybrid promotor

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 583
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 583
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 583
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


By combining multiple operator sites in a promoter region, a “hybrid promoter” can be created that responds to multiple input signals. This creates a logic transcriptional AND gate, of which the dynamic range can be further tuned by changing -35 and -10 sites. This E. coli promoter, Plux/lac, was developed by Chen et al. (2018) [1] and contains operator sites for LacI repression and LuxR activation. Transcription is fully activated when LacI is absent and LuxR is present. Different -10 sites “E” and “F” influence the transcription rate when only one of the two input signals is present (Figure 1).


T--Wageningen_UR--wetlab_MKS_HP.png

<a href="https://2021.igem.org/wiki/index.php?title=Team:Wageningen">BB2<a/>

References

1. Y. Chen, J. M. L. Ho, D. L. Shis, et al., Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors. Nat. Commun. 9, 1–8 (2018).