Difference between revisions of "Part:BBa K3714004"
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<partinfo>BBa_K3714004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3714004 SequenceAndFeatures</partinfo> | ||
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− | By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig. | + | By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.2). Our aptazyme exhibits a 2-fold change of cleavage proportion at ~1mg/ml (0.125x dilution of saturated solution at room temperature) while the origin apatazyme (BBa_J119449) shows only 1.3-fold change. |
− | <center>[[File: | + | <center>[[File:T--SJTU-BioX-Shanghai--improve-5.jpg]]</center> |
− | <center><b>Figure | + | <center><b>Figure 2 | Improvement of an existing theophylline aptazyme. a.</b>Cleavage properties of two aptazymes under different theophylline concentration. The sequences were synthesized and transcribed <i>in vitro</i>. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. <b>b. </b>A diagram showing the intensity percentage of the cleaved band of each lane in <b>a</b>. Intensity was measured by ImageJ.</center> |
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Revision as of 15:22, 21 October 2021
Improved theophylline biosensor
An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.2). Our aptazyme exhibits a 2-fold change of cleavage proportion at ~1mg/ml (0.125x dilution of saturated solution at room temperature) while the origin apatazyme (BBa_J119449) shows only 1.3-fold change.