Difference between revisions of "Part:BBa K3714004"
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<partinfo>BBa_K3714004 short</partinfo> | <partinfo>BBa_K3714004 short</partinfo> | ||
| − | An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (<a href="https://parts.igem.org/Part:BBa_J119449">BBa_J119449</a>). | + | An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (<html><a href="https://parts.igem.org/Part:BBa_J119449">BBa_J119449</a></html>). |
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Revision as of 13:12, 21 October 2021
Improved theophylline biosensor
An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1). Our aptazyme exhibits a 2-fold change of cleavage proportion under 10μM at ~1mg/ml (0.125x dilution of saturated solution at room temperature) while the origin apatazyme (BBa_J119449) shows only 1.3-fold change.
