Difference between revisions of "Part:BBa K4023003"
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This is a bicistronic gene containing a [https://parts.igem.org/Part:BBa_K4023001 glpF], a [https://parts.igem.org/Part:BBa_K4023002 ribosome binding site] and a [https://parts.igem.org/Part:BBa_K4023000 modified MTIA]. This gene is designed to coexpress the glycerol uptake facilitator protein (coded by glpF) with the modified metallothionein IA protein. The glycerol uptake facilitator protein transports arsenite into E.coli. This could help to remove the bottleneck IN As III uptake, potentially improving the efficiency of arsenite remediation<sup>1</sup>. Thus This bicistronic gene has been optimized to express the glycerol uptake facilitator protein and modified metallothionein IA protein for increased remediation of As III from the environment.  
 
This is a bicistronic gene containing a [https://parts.igem.org/Part:BBa_K4023001 glpF], a [https://parts.igem.org/Part:BBa_K4023002 ribosome binding site] and a [https://parts.igem.org/Part:BBa_K4023000 modified MTIA]. This gene is designed to coexpress the glycerol uptake facilitator protein (coded by glpF) with the modified metallothionein IA protein. The glycerol uptake facilitator protein transports arsenite into E.coli. This could help to remove the bottleneck IN As III uptake, potentially improving the efficiency of arsenite remediation<sup>1</sup>. Thus This bicistronic gene has been optimized to express the glycerol uptake facilitator protein and modified metallothionein IA protein for increased remediation of As III from the environment.  
  
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===Usage and Biology===
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===References===
 
===References===
 
1. Singh, S., Mulchandani, A., & Chen, W. (2008). Highly selective and rapid arsenic removal by metabolically engineered Escherichia coli cells expressing Fucus vesiculosus metallothionein. Applied and environmental microbiology, 74(9), 2924-2927.
 
1. Singh, S., Mulchandani, A., & Chen, W. (2008). Highly selective and rapid arsenic removal by metabolically engineered Escherichia coli cells expressing Fucus vesiculosus metallothionein. Applied and environmental microbiology, 74(9), 2924-2927.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:21, 21 October 2021


glpF-rbs-modified MTIA

This is a bicistronic gene containing a glpF, a ribosome binding site and a modified MTIA. This gene is designed to coexpress the glycerol uptake facilitator protein (coded by glpF) with the modified metallothionein IA protein. The glycerol uptake facilitator protein transports arsenite into E.coli. This could help to remove the bottleneck IN As III uptake, potentially improving the efficiency of arsenite remediation1. Thus This bicistronic gene has been optimized to express the glycerol uptake facilitator protein and modified metallothionein IA protein for increased remediation of As III from the environment.



References

1. Singh, S., Mulchandani, A., & Chen, W. (2008). Highly selective and rapid arsenic removal by metabolically engineered Escherichia coli cells expressing Fucus vesiculosus metallothionein. Applied and environmental microbiology, 74(9), 2924-2927.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183