Difference between revisions of "Part:BBa K3805209"

Revision as of 10:45, 21 October 2021

Bi-stable Switch

The bistable switch is composed of trc promoter(BBa_K3805208))[1], P2 promoter(BBa_I746104))[2], lacI- LVA (BBa_C0012))[3]and ompT-mcherry(BBa_K3805886))[4]. At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[5]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1287
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3805209 short</partinfo>
-The bistable switch is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886]. At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[https://parts.igem.org/Part:BBa_K3805508]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.
+The bistable switch is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886]. At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[https://parts.igem.org/Part:BBa_K3805525]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.
 <!-- Add more about the biology of this part here