Difference between revisions of "Part:BBa K3815012"
| Line 21: | Line 21: | ||
This part was inserted in L4440. The L4440 has two t7 promoters, and this parts are transcribed from both sides. | This part was inserted in L4440. The L4440 has two t7 promoters, and this parts are transcribed from both sides. | ||
<br> | <br> | ||
| − | + | <h3><font size="3"></font> Reference </h3> | |
| + | 1 Andongma, A.A., Greig, C., Dyson, P.J., Flynn, N., and Whitten, M.M.A. (2020). Optimization of dietary RNA interference delivery to western flower thrips Frankliniella occidentalis and onion thrips Thrips tabaci. Arch. Insect Biochem. Physiol. 103, e21645. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3815012 parameters</partinfo> | <partinfo>BBa_K3815012 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Revision as of 11:02, 21 October 2021
Part of the TrxZ gene of A. thaliana to synthesize dsRNA for RNAi
This is a section of TrxZ gene of Arabidopsis thaliana to synthesize dsRNA for RNAi. To product dsRNA, this sequence is inserted in L4440 plasmid, and transformed into HT115(DE3).
purpose
Trx-Z is a gene responsible for chloroplast formation during early development in Arabidopsis thaliana. Silencing this genes is whitening leaf of Arabidopsis thaliana[1].
Usage and Biology
RNAi
RNAi (RNA interference) is a process in which externally introduced dsRNA suppresses the expression of genes that have complementary sequences to the dsRNA.
L4440
L4440 is a plasmid vector having two convergent T7 promoters adjacent to the multi-cloning site. By inserting a portion of the target gene sequence into the multi-cloning site of this plasmid, the target sequence is transcribed from both sides, and dsRNA can be obtained when both parts anneal.
HT115(DE3)
HT115 (DE3) is an RNase III-deficient E. coli strain that has been modified to express T7 RNA polymerase from an IPTG-inducible promoter. It lacks dsRNA-specific RNase III, which allows it to produce high levels of specific dsRNA. These attributes allow HT115 (DE3) to be a promising strain for the preparation of large amounts of viral dsRNA in vivo.
Sequence and features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 442
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
cloning
This part was inserted in L4440. The L4440 has two t7 promoters, and this parts are transcribed from both sides.
Reference
1 Andongma, A.A., Greig, C., Dyson, P.J., Flynn, N., and Whitten, M.M.A. (2020). Optimization of dietary RNA interference delivery to western flower thrips Frankliniella occidentalis and onion thrips Thrips tabaci. Arch. Insect Biochem. Physiol. 103, e21645.