Difference between revisions of "Part:BBa K4023001"
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glpF codes for a 28kDa<sup>1</sup> transmembrane glycerol uptake facilitator protein found in E.coli strain. glpF protein catalyze transmembrane diffusion of glycerol and some linear polyhydric alcohols in E.coli<sup>1</sup>. glpF protein also allows Arsenite to be transported into E.coli. This is as Arsenite is protonated at physiological pH and forms As(OH)3 as a major species. As(OH)3 is chemically analogous to glycerol<sup>2</sup>.
 
glpF codes for a 28kDa<sup>1</sup> transmembrane glycerol uptake facilitator protein found in E.coli strain. glpF protein catalyze transmembrane diffusion of glycerol and some linear polyhydric alcohols in E.coli<sup>1</sup>. glpF protein also allows Arsenite to be transported into E.coli. This is as Arsenite is protonated at physiological pH and forms As(OH)3 as a major species. As(OH)3 is chemically analogous to glycerol<sup>2</sup>.
  
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The glpF sequence was taken from [https://parts.igem.org/Part:BBa_K190028 iGEM09_Groningen]. We optimized the sequence using IDT optimization tool and Benchling for expression within an E.coli host.  
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The glpF sequence was obtained from [https://parts.igem.org/Part:BBa_K190028 iGEM09_Groningen]. We optimized the sequence using IDT optimization tool and Benchling for expression within an E.coli host.  
  
 
===References===
 
===References===

Revision as of 22:54, 21 October 2021


Optimized glpF

glpF codes for a 28kDa1 transmembrane glycerol uptake facilitator protein found in E.coli strain. glpF protein catalyze transmembrane diffusion of glycerol and some linear polyhydric alcohols in E.coli1. glpF protein also allows Arsenite to be transported into E.coli. This is as Arsenite is protonated at physiological pH and forms As(OH)3 as a major species. As(OH)3 is chemically analogous to glycerol2.

The glpF sequence was obtained from iGEM09_Groningen. We optimized the sequence using IDT optimization tool and Benchling for expression within an E.coli host.

References

1. Fu, D., Libson, A., & Stroud, R. (2002, April). The structure of G1pF, a glycerol conducting channel. In Ion Channels: From Atomic Resolution Physiology to Functional Genomics: Novartis Foundation Symposium 245 (Vol. 245, pp. 51-65). Chichester, UK: John Wiley & Sons, Ltd.

2. Bhattacharjee, H., Mukhopadhyay, R., Thiyagarajan, S., & Rosen, B. P. (2008). Aquaglyceroporins: ancient channels for metalloids. Journal of biology, 7(9), 1-6.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183