Difference between revisions of "Part:BBa K3806012:Design"
 
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===Design Notes===
 
===Design Notes===
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<HTML><a href="https://parts.igem.org/Part:BBa_K3806012" target="_blank"><b>BBa_K3806012</b></a></HTML> contains a codon optimized <i>lacZ</i> gene for optimal expression in <i>E.coli</i>. The codon optimization was performed with the Twist Codon Optimizer Tool. This part is used in <HTML><a href="https://parts.igem.org/Part:BBa_K3806014" target="_blank"><b>BBa_K3806014</b></a></HTML> and <HTML><a href="https://parts.igem.org/Part:BBa_K3806016" target="_blank"><b>BBa_K3806016</b></a></HTML> as reporter gene within a aptazyme-regulated genetic circuit that enables a ligand-dependent colorimetric read-out.
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<HTML><a href="https://parts.igem.org/Part:BBa_K3806012" target="_blank"><b>BBa_K3806012</b></a></HTML> contains a codon optimized <i>lacZ</i> gene for optimal expression in <i>E. coli</i>. The codon optimization was performed with the Twist Codon Optimizer Tool.

Latest revision as of 10:07, 21 October 2021


lacZ reporter gene codon optimized for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2193
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K3806012 contains a codon optimized lacZ gene for optimal expression in E. coli. The codon optimization was performed with the Twist Codon Optimizer Tool.