Difference between revisions of "Part:BBa K3711053"
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A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it. | A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it. | ||
<h1>'''Molecular cloning'''</h1> | <h1>'''Molecular cloning'''</h1> | ||
| − | + | [[File:T--HUST-China--53-1.png|400px|thumb|center|Fig1. Plasmid construction and colony PCR results of Panb1-α factor-FMO-AOX1 Terminator and Pynr071c-α factor-ROX1-AOX1 Terminator transformed E.coli ]] | |
Latest revision as of 09:36, 21 October 2021
Pynr071c-α factor-ROX1-AOX1 Terminator
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2711
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1630
Illegal SapI site found at 1889
Description
This is a composite part for extracellular expression of ROX1. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, the signal peptide, α-factor , is used to express ROX1 outside of the cell.
Usage and Biology
A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it.
