Difference between revisions of "Part:BBa K3895016"
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==Experiment details== | ==Experiment details== | ||
• Throughput: 2500<br> | • Throughput: 2500<br> | ||
| − | • Organism: E. coli | + | • Organism: E. coli<br> |
| − | • Strain type: BL21(DE3) | + | • Strain type: BL21(DE3)<br> |
| − | • Temperature: 37°C | + | • Temperature: 37°C<br> |
| − | • Media: 250 ml LB | + | • Media: 250 ml LB<br> |
| − | • Period of time: 16 hours | + | • Period of time: 16 hours<br> |
| − | • Plasmid backbone: pSB1C3 | + | • Plasmid backbone: pSB1C3<br> |
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Revision as of 08:29, 21 October 2021
Optimized keratinase KerA
Introduction
This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3). The KERA (BBa_K1717171) is a basic part comprised from a synthesized KERA protein coding sequence. The gene can be expressed in cells by looking for clear areas of cell growth on AGAR plates of skimmed milk, and by observing keratin-containing substrates (feathers, hair, chemosynthetic keratin, etc.) growing with cells.
Plasmid Construction
Experiment details
• Throughput: 2500
• Organism: E. coli
• Strain type: BL21(DE3)
• Temperature: 37°C
• Media: 250 ml LB
• Period of time: 16 hours
• Plasmid backbone: pSB1C3
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 326
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 587
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1369
Illegal SapI site found at 957
Illegal SapI site found at 1260