Difference between revisions of "Part:BBa K3765008"
 
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<partinfo>BBa_K3765008 short</partinfo>
 
<partinfo>BBa_K3765008 short</partinfo>
  
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Phosphotriesterase enzyme from ''Agrobacterium tumefaciens'', which degrades organophosphates which are triesters of phosphoric acid. Unlike the previous OpdA part on the Registry [https://parts.igem.org/Part:K215090 BBa_K215090], this part also encodes the  17 amino acid long tail at the C-terminus of the enzyme which may be useful for construction of C-terminal fusion proteins using this part.
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Phosphotriesterase enzyme from ''Agrobacterium tumefaciens'', which degrades organophosphates which are triesters of phosphoric acid. Unlike the previous OpdA part on the Registry [https://parts.igem.org/Part:BBa_K215090 BBa_K215090], this part also encodes the  17 amino acid long tail at the C-terminus of the enzyme which may be useful for construction of C-terminal fusion proteins using this part.
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 07:08, 21 October 2021


OpdA

Phosphotriesterase enzyme from Agrobacterium tumefaciens, which degrades organophosphates which are triesters of phosphoric acid. Unlike the previous OpdA part on the Registry BBa_K215090, this part also encodes the 17 amino acid long tail at the C-terminus of the enzyme which may be useful for construction of C-terminal fusion proteins using this part.

Usage and Biology

This part was of major importance in our project. The OpdA enzyme can degrade a wide range of organophosphates and since our project is aimed at building a filter to fight organophosphate pollution, we constructed an OpdA-SpyTag002 fusion protein to functionalize a microcrystalline cellulose sheet-based filter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]