Difference between revisions of "Part:BBa K3745005"

Latest revision as of 01:34, 22 October 2021

EC6015/Bujard RBS for biosensor

This part is the modified RBS for our biosensor. Ailurus Biotechnology helped us to fine-tune its strength to ensure the rhamnose yield is within the dynamic range of the rhamnose-inducible genetic circuit.

Characterization

The basic Characterization information of this part is listed below:

• Throughput: 2500

• Organism: E. coli

• Strain type: BL21(DE3)

• Temperature: 30°C

• Media: 250 ml LB + 0.1g/L rhamnose (w./v.)

• Period of time: 12 hours

• Plasmid backbone: pSB1C3

Under this condition, the expression of antibiotics resistance gene significantly changes with the concentration of rhamnose.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 31
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 31
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 31
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 31
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 31
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This part is the modified RBS for our biosensor. Ailurus Biotechnology helped us to fine-tune its strength to ensure the rhamnose yield is within the dynamic range of the rhamnose-inducible genetic circuit.
+===Characterization===
+The basic Characterization information of this part is listed below:
+• Throughput: 2500
+• Organism: E. coli
+• Strain type: BL21(DE3)
+• Temperature: 30°C
+• Media: 250 ml LB + 0.1g/L rhamnose (w./v.)
+• Period of time: 12 hours
+• Plasmid backbone: pSB1C3
+Under this condition, the expression of antibiotics resistance gene significantly changes with the concentration of rhamnose.
 <!-- Add more about the biology of this part here