Difference between revisions of "Part:BBa K3739024"
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1. | After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1. | ||
| − | + | ::'''Fig. 1.''' Gene circuit and agarose gel electrophoresis. (A) Gene circuit of LamB-LCIKR-2-GFP (BBa_K3739024). (B)Target bands of LamB-LCIKR-2-GFP (black arrow, 2300 bp). | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 17:53, 21 October 2021
LamB-LC1KR2-GFP
We anchor LCI-KR2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene. We use BBa_K3739051 to construct the expression system and anchor LCI-KR2 on the surface of VnDX.
Biology
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCI-KR2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCI-KR2 is fused at C terminal with LamB so that LCI-KR2 can be displayed on the surface of VnDX.
Characterization
1. Identification
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
- Fig. 1. Gene circuit and agarose gel electrophoresis. (A) Gene circuit of LamB-LCIKR-2-GFP (BBa_K3739024). (B)Target bands of LamB-LCIKR-2-GFP (black arrow, 2300 bp).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 528
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 676
Illegal SapI.rc site found at 1432