Difference between revisions of "Part:BBa K176007"
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<partinfo>BBa_K176007 short</partinfo> | <partinfo>BBa_K176007 short</partinfo> | ||
It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002. | It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002. | ||
| − | SpeI-RBS-mRFP-Terminator-PstI-pSB1A3-prefix | + | |
| + | SpeI-RBS-mRFP-Terminator-NotI-PstI-pSB1A3-prefix | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:05, 16 June 2009
pSB1A3 with the suffix of J61002 (mRFP)
It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002.
SpeI-RBS-mRFP-Terminator-NotI-PstI-pSB1A3-prefix
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 885 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3003
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal NotI site found at 878
Illegal NotI site found at 3009 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3003 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3003
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 885 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3003
Plasmid lacks a suffix.
Illegal XbaI site found at 3018
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal AgeI site found at 581
Illegal AgeI site found at 693 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2042