Difference between revisions of "Help:Plasmid Backbone Availability 2009"
Line 18: Line 18:
  
 
|- valign='top'
 
|- valign='top'
−
|'''BBa_I51020''' || YES || YES || NO ||
+
|'''pSB1A3''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 2, well 10G [https://parts.igem.org/partsdb/get_part.cgi?id=4586&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I732205 BBa_I732205]
 +
|-
 +
|'''pSB1AC3''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 2, well 22M [https://parts.igem.org/partsdb/get_part.cgi?id=5485&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_M1001 BBa_M1001]
 
|-
 
|-
−
|'''BBa_I716101''' || YES [?] || NO || NO ||
+
|'''pSB1AK3''' || NO || NO || Kit Plate 3, well 20A [https://parts.igem.org/partsdb/get_part.cgi?id=4942&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K143053 BBa_K143053]
 
|-
 
|-
−
|'''BBa_J63009''' || YES || YES [?] || Kit Plate 3, well 2M [https://parts.igem.org/partsdb/get_part.cgi?id=7521&show=1 [more]]|| [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165018 BBa_K165018]
+
|'''pSB1AT3''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 3, well 1B [https://parts.igem.org/partsdb/get_part.cgi?id=5483&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K115001 BBa_K115001]
−
|-
+
−
|'''BBa_J63010''' || NO || NO || Kit Plate 3, well 2K [https://parts.igem.org/partsdb/get_part.cgi?id=7585&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165001 BBa_K165001]
+
 
|-
 
|-
 
|'''pSB1A10''' || NO || NO || NO ||
 
|'''pSB1A10''' || NO || NO || NO ||
 
|-
 
|-
−
|'''pSB1A3''' || YES || YES || Kit Plate 2, well 10G [https://parts.igem.org/partsdb/get_part.cgi?id=4586&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I732205 BBa_I732205]
+
|'''pSB4A5''' || NO || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO ||
−
|-
+
−
|'''pSB4A5''' || NO || YES || NO ||
+
 
|-  
 
|-  
 
|'''pSB6A1''' || NO || NO || Kit Plate 2, well 14G [https://parts.igem.org/partsdb/get_part.cgi?id=10245&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I739001 BBa_I739001]
 
|'''pSB6A1''' || NO || NO || Kit Plate 2, well 14G [https://parts.igem.org/partsdb/get_part.cgi?id=10245&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I739001 BBa_I739001]
 
|-
 
|-
−
|'''pSB1AC3''' || YES [?] || YES [?] || Kit Plate 2, well 22M [https://parts.igem.org/partsdb/get_part.cgi?id=5485&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_M1001 BBa_M1001]
+
|'''pSB1C3''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO ||
−
|-
+
−
|'''pSB1AK3''' || NO || NO || Kit Plate 3, well 20A [https://parts.igem.org/partsdb/get_part.cgi?id=4942&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K143053 BBa_K143053]
+
−
|-
+
−
|'''pSB1AK8''' || YES [?] || NO || Kit Plate 2, well 13A [https://parts.igem.org/partsdb/get_part.cgi?id=7524&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I712006 BBa_I712006]
+
−
|-
+
−
|'''pSB1AT3''' || YES || YES [?] || Kit Plate 3, well 1B [https://parts.igem.org/partsdb/get_part.cgi?id=5483&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K115001 BBa_K115001]
+
−
|-
+
−
|'''pSB1C3''' || YES || YES || NO ||
+
 
|-  
 
|-  
−
|'''pSB3C5''' || NO || YES || NO ||
+
|'''pSB3C5''' || NO || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO ||
 
|-  
 
|-  
−
|'''pSB4C5''' || NO || YES || Kit Plate 3, well 5F [https://parts.igem.org/partsdb/get_part.cgi?id=8151&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K098010 BBa_K098010]
+
|'''pSB4C5''' || NO || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 3, well 5F [https://parts.igem.org/partsdb/get_part.cgi?id=8151&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K098010 BBa_K098010]
 
|-  
 
|-  
−
|'''pSB1K3''' || YES [?] || NO || NO ||
+
|'''pSB1K3''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || NO ||
 
|-
 
|-
 
|'''pSB2K3''' || NO || NO || Kit Plate 2, well 11B [https://parts.igem.org/partsdb/get_part.cgi?id=4584&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_E0033 BBa_E0033]
 
|'''pSB2K3''' || NO || NO || Kit Plate 2, well 11B [https://parts.igem.org/partsdb/get_part.cgi?id=4584&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_E0033 BBa_E0033]
Line 54: Line 44:
 
|'''pSB3K3''' || NO || NO || Kit Plate 2, well 15L [https://parts.igem.org/partsdb/get_part.cgi?id=4739&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I714891 BBa_I714891]
 
|'''pSB3K3''' || NO || NO || Kit Plate 2, well 15L [https://parts.igem.org/partsdb/get_part.cgi?id=4739&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I714891 BBa_I714891]
 
|-
 
|-
−
|'''pSB4K5''' || YES [?] || NO || Kit Plate 2, well 17H [https://parts.igem.org/partsdb/get_part.cgi?id=8325&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_T9003 BBa_T9003]
+
|'''pSB4K5''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || Kit Plate 2, well 17H [https://parts.igem.org/partsdb/get_part.cgi?id=8325&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_T9003 BBa_T9003]
 
|-
 
|-
−
|'''pSB1T3''' || YES [?] || NO || NO ||
+
|'''pSB1AK8''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || Kit Plate 2, well 13A [https://parts.igem.org/partsdb/get_part.cgi?id=7524&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I712006 BBa_I712006]
 
|-
 
|-
−
|'''pSB3T5''' || YES [?] || YES [?] || Kit Plate 2, well 19A [https://parts.igem.org/partsdb/get_part.cgi?id=8615&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I715045 BBa_I715045]
+
|'''pSB1T3''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || NO ||
 +
|-
 +
|'''pSB3T5''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 2, well 19A [https://parts.igem.org/partsdb/get_part.cgi?id=8615&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I715045 BBa_I715045]
 
|-
 
|-
 
|'''pSB4T5''' || NO || NO || NO ||
 
|'''pSB4T5''' || NO || NO || NO ||
 +
|-
 +
|'''BBa_I51020''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO ||
 +
|-
 +
|'''BBa_I716101''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || NO ||
 +
|-
 +
|'''BBa_J63009''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 3, well 2M [https://parts.igem.org/partsdb/get_part.cgi?id=7521&show=1 [more]]|| [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165018 BBa_K165018]
 +
|-
 +
|'''BBa_J63010''' || NO || NO || Kit Plate 3, well 2K [https://parts.igem.org/partsdb/get_part.cgi?id=7585&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165001 BBa_K165001]
 
|-
 
|-
 
|}
 
|}

Revision as of 14:33, 15 June 2009

**EDITING IN PROGRESS**

The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.


  • YES - The plasmid is available and correct
  • YES[?] - The plasmid is available and correct, with the exception of one inconsistency in the QC results which should not be an issue
  • NO - The plasmid is either unavailable or we have found that it carries an error


Plasmid J04450 P1010 Alt. Location Insert (click for QC)
pSB1A3 YES
[ Kit Plate 1, well ] 		YES
[ Kit Plate 1, well ] 		Kit Plate 2, well 10G [more] BBa_I732205
pSB1AC3 YES [?]
[ Kit Plate 1, well ] 		YES [?]
[ Kit Plate 1, well ] 		Kit Plate 2, well 22M [more] BBa_M1001
pSB1AK3 NO NO Kit Plate 3, well 20A [more] BBa_K143053
pSB1AT3 YES
[ Kit Plate 1, well ] 		YES [?]
[ Kit Plate 1, well ] 		Kit Plate 3, well 1B [more] BBa_K115001
pSB1A10 NO NO NO
pSB4A5 NO YES
[ Kit Plate 1, well ] 		NO
pSB6A1 NO NO Kit Plate 2, well 14G [more] BBa_I739001
pSB1C3 YES
[ Kit Plate 1, well ] 		YES
[ Kit Plate 1, well ] 		NO
pSB3C5 NO YES
[ Kit Plate 1, well ] 		NO
pSB4C5 NO YES
[ Kit Plate 1, well ] 		Kit Plate 3, well 5F [more] BBa_K098010
pSB1K3 YES [?]
[ Kit Plate 1, well ] 		NO NO
pSB2K3 NO NO Kit Plate 2, well 11B [more] BBa_E0033
pSB3K3 NO NO Kit Plate 2, well 15L [more] BBa_I714891
pSB4K5 YES [?]
[ Kit Plate 1, well ] 		NO Kit Plate 2, well 17H [more] BBa_T9003
pSB1AK8 YES [?]
[ Kit Plate 1, well ] 		NO Kit Plate 2, well 13A [more] BBa_I712006
pSB1T3 YES [?]
[ Kit Plate 1, well ] 		NO NO
pSB3T5 YES [?]
[ Kit Plate 1, well ] 		YES [?]
[ Kit Plate 1, well ] 		Kit Plate 2, well 19A [more] BBa_I715045
pSB4T5 NO NO NO
BBa_I51020 YES
[ Kit Plate 1, well ] 		YES
[ Kit Plate 1, well ] 		NO
BBa_I716101 YES [?]
[ Kit Plate 1, well ] 		NO NO
BBa_J63009 YES
[ Kit Plate 1, well ] 		YES [?]
[ Kit Plate 1, well ] 		Kit Plate 3, well 2M [more] BBa_K165018
BBa_J63010 NO NO Kit Plate 3, well 2K [more] BBa_K165001


  • If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to make the construction plasmid. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.


We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.