Difference between revisions of "Part:BBa K3725010:Experience"
| Line 6: | Line 6: | ||
<br> | <br> | ||
<br> | <br> | ||
| − | <center>[[File:T--Lambert GA-- | + | <center>[[File:T--Lambert GA--PhytoIPTGFluorescenceDataGraph.jpeg|500px]]</center> |
<br> | <br> | ||
<center><I>Figure 2: This graph compares the mean fluorescence per optical density values of the P. cryptogea toehold switch, positive control (pUC19), and the dual plasmid. The toehold switch and positive control have very low fluorescence per optical density values, whereas the dual plasmid has a higher fluorescence per optical density value, that is significantly different from the other two groups..</I></center> | <center><I>Figure 2: This graph compares the mean fluorescence per optical density values of the P. cryptogea toehold switch, positive control (pUC19), and the dual plasmid. The toehold switch and positive control have very low fluorescence per optical density values, whereas the dual plasmid has a higher fluorescence per optical density value, that is significantly different from the other two groups..</I></center> | ||
Revision as of 02:57, 21 October 2021
Experience
The T7 Phytophthora Trigger (BBa_K3725040) and the Phytophthora Toehold w/ GFP Reporter are intended to be compatible with each other and used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was greater and had a statistically significant difference in comparison to cells transformed with only the toehold part and pUC19 plasmid.
Applications of BBa_K3725010
User Reviews
UNIQf4490024f5bd0c8d-partinfo-00000000-QINU UNIQf4490024f5bd0c8d-partinfo-00000001-QINU