Difference between revisions of "Part:BBa K3714002"
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<partinfo>BBa_K3714002 short</partinfo>
 
<partinfo>BBa_K3714002 short</partinfo>
  
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The template of aptazyme gard-337 with two probes (binding BBa_K3714007 and BBa_K3714008) on both terminal for lateral flow assay.
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The template of aptazyme gard-337 with two probes (binding <html><a href="https://parts.igem.org/Part:BBa_K3714007">BBa_K3714007</a></html> and <html><a href="https://parts.igem.org/Part:BBa_K3714008">BBa_K3714008</a></html>) on both terminal for lateral flow assay (LFA). It could be used as a biosensor of gardiquimod to verify our design on a colloidal gold based detection platform (Figure.1).
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<center>[[File:K3714002-0.png]]</center>
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<center><b>Figure.1 | Design of lateral flow assay. </b> T7 transcription products with or without certain molecule are used as input of LFA. When the certain molecule exists, positive signal could be seen on test line. T line, test line. C line, control line.</center>
 
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===Usage and Biology===
 
===Usage and Biology===
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<center>[[File:K3714002-1.png]]</center>
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<center><b>Figure.3 | Verification of cleavage property of aptazyme. </b> T7 transcription products were loaded on a denatured PAGE to determine its cleave fraction.</center>
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<center>[[File:K3714002-2.png]]</center>
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<center><b>Figure.3 | Verification of lateral flow assay. </b> T7 transcription product with a target concentration gradient were used as input of LFA.</center>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3714002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3714002 SequenceAndFeatures</partinfo>
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<center>[[File:K3714002.png]]</center>
 
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<center><b>Figure.1 | Verification of lateral flow assay. </b> T7 transcription product with a target concentration gradient were used as input of LFA. T, test line. C, control line. </center>
 
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Revision as of 02:50, 22 October 2021


Biosensor gard-337 for lateral flow assay

The template of aptazyme gard-337 with two probes (binding BBa_K3714007 and BBa_K3714008) on both terminal for lateral flow assay (LFA). It could be used as a biosensor of gardiquimod to verify our design on a colloidal gold based detection platform (Figure.1).

K3714002-0.png
Figure.1 | Design of lateral flow assay. T7 transcription products with or without certain molecule are used as input of LFA. When the certain molecule exists, positive signal could be seen on test line. T line, test line. C line, control line.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]