Difference between revisions of "Part:BBa K4046780"
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<partinfo>BBa_K4046780 short</partinfo> | <partinfo>BBa_K4046780 short</partinfo> | ||
| − | This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and tdTomato gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations'' section. | + | This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and <i>tdTomato</i> gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations'' section. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:16, 21 October 2021
CMV - BS #2 - BS #2 - BS #2 - Kozak - tdTomato - bghA
This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and tdTomato gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations section. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1040
Illegal PstI site found at 1766 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1040
Illegal PstI site found at 1766 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1040
Illegal PstI site found at 1766 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1040
Illegal PstI site found at 1766 - 1000COMPATIBLE WITH RFC[1000]