Difference between revisions of "Part:BBa K3788017"

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<partinfo>BBa_K3788017 short</partinfo>
 
<partinfo>BBa_K3788017 short</partinfo>
  
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Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis.  
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<p>This part is constituted from  -35 and -10 boxes, with a LexA box and the beggining of the CDS of <i>caa</i>.
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The LexA box is a sequence that allows the binding of a tetramere of LexA that represses the trascription of the <i>caa-cal</i> operon.</p>
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<p>This repression can be stopped when the SOS response is activated, due to DNA damage that activate RecA production that induce LexA's depolymersation.</p>
  
  
  
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===Description of the part===
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===Role in natural environment===
  
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<p>This part is constituted from  -35 and -10 boxes, with a LexA box and the beggining of the CDS of <i>caa</i>.
+
Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis.  
−
The LexA box is a sequence that allows the binding of a tetramere of LexA that represses the trascription of the <i>caa-cal</i> operon.</p>
+
−
<p>This repression can be stopped when the SOS response is activated, due to DNA damage that activate RecA production that induce LexA's depolymersation.</p>
+
  
  

Revision as of 21:55, 20 October 2021


lexA-repressed promoter

This part is constituted from -35 and -10 boxes, with a LexA box and the beggining of the CDS of caa. The LexA box is a sequence that allows the binding of a tetramere of LexA that represses the trascription of the caa-cal operon.

This repression can be stopped when the SOS response is activated, due to DNA damage that activate RecA production that induce LexA's depolymersation.


Role in natural environment

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]