Difference between revisions of "Part:BBa K678020"
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p> | ||
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Revision as of 19:11, 20 October 2021
mCherry-module
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
Supplementary Experiment
Group: CSU_CHINA 2021
Author: Xingjun Zhao
mCherry is a monomer red fluorescent protein. Experiments proved that when it is combined with exogenous protein on both C side and N side, the activity of fluorescent and the function of combined proteinare not effected and it can be used together with multi types of fluorescent proteins. Due to the phosphorylation pathway is relatively long, we have added mCherry at the bottom as report gene to prove that our pathway works and is regulated by insulin.
Fig.1 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 367
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 367
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 367
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 367
- 1000COMPATIBLE WITH RFC[1000]