Difference between revisions of "Part:BBa K4006001"

Revision as of 19:09, 20 October 2021

arsC

This part contained a protein coding sequence for arsenate reductase that has been codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. Arsenate reductase catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)]. Using 2 µM arsenate at a substrate, the Kcat of the protein has been recorded as 4.5 min(-1).

We were able to clone this construct into our plasmid and select for transformed E. coli colonies.

Digestion of the miniprepped DNA with SOMETHING and SOMETHING should result in X bands of approximate sizes X and Y.

Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae.

Sequence and Features